Table 1.
Group | Wet weight (mg) | Water content (%) | Diameter (mm) | Thickness (mm) | Cells (×10^6) | ||
---|---|---|---|---|---|---|---|
Phase 1 | |||||||
Control | 32.9 ± 1.0AB | 89.7 ± 2.3A | 6.18 ± 0.14A | 0.81 ± 0.15A | 3.72 ± 0.45AB | ||
Week 1 Treatment | PTH | 33.9 ± 1.0A | 89.4 ± 0.6A | 6.09 ± 0.09A | 0.77 ± 0.05A | 3.57 ± 0.20AB | |
T3 | 17.1 ± 0.5C | 85.0 ± 0.8B | 5.66 ± 0.05B | 0.54 ± 0.07B | 3.10 ± 0.11BC | ||
T4 | 17.8 ± 0.7C | 85.3 ± 1.4B | 5.80 ± 0.09B | 0.53 ± 0.02B | 2.87 ± 0.24C | ||
Week 3 Treatment | PTH | 31.5 ± 1.4B | 88.3 ± 0.5A | 6.28 ± 0.12A | 0.69 ± 0.05A | 3.54 ± 0.30AB | |
T3 | 32.8 ± 1.0AB | 87.8 ± 0.6A | 6.24 ± 0.05A | 0.72 ± 0.05A | 3.60 ± 0.26AB | ||
T4 | 33.0 ± 1.3AB | 88.3 ± 0.8A | 6.18 ± 0.09A | 0.74 ± 0.03A | 3.71 ± 0.30A | ||
Phase 2 | |||||||
No T3 Treatment | Control | 15.4 ± 0.6a | 89.5 ± 0.5a | 5.65 ± 0.13ab | 0.56 ± 0.05ab | 2.85 ± 0.27a | |
PTH Week 2 | 16.6 ± 1.5a | 89.6 ± 0.6a | 5.71 ± 0.10ab | 0.61 ± 0.04a | 3.00 ± 0.21a | ||
PTH Week 3 | 16.1 ± 0.5a | 89.4 ± 0.3a | 5.71 ± 0.08ab | 0.60 ± 0.03a | 3.01 ± 0.20a | ||
PTH Week 2-4 | 16.2 ± 0.5a | 89.1 ± 0.4a | 5.80 ± 0.09a | 0.85 ± 0.03ab | 2.74 ± 0.47a | ||
Week 1 T3 Treatment | No PTH | 13.4 ± 0.9b | 84.9 ± 1.0b | 5.67 ± 0.12ab | 0.55 ± 0.08ab | 2.71 ± 0.24a | |
PTH Week 2 | 14.0 ± 0.7b | 84.9 ± 0.6b | 5.69 ± 0.23ab | 0.57 ± 0.04ab | 2.98 ± 0.15a | ||
PTH Week 3 | 13.1 ± 0.8b | 85.6 ± 0.9b | 5.53 ± 0.09b | 0.59 ± 0.11ab | 2.91 ± 0.19a | ||
PTH Week 2-4 | 13.3 ± 0.6b | 84.7 ± 0.6b | 5.60 ± 0.16ab | 0.49 ± 0.04b | 2.72 ± 0.17a |
Phase 1 and 2 construct morphology and cellular content. Phase 2 used a lower seeding density (for example, 4.57 versus 5.32 million cells in Phase 1) and resulted in smaller constructs. Letters represent the statistical results of a one-way and two-way ANOVA for Phase 1 and 2, respectively. Groups not connected by the same letter are statistically significant. Data are represented as mean ± standard deviation.