Figure 7.
miR-146a suppresses interferon-stimulated genes. Effect of miR-146a overexpression on JEV-induced expression of interferon-stimulated genes. The levels of IFIT-1 and IFIT-2 were determined by RT-PCR. The CT values were normalized by GAPDH levels, and fold change was calculated by 2−∆∆C T method. (A,C) Graph bars showing decreased expression of JEV-induced IFIT-1 (A) and IFIT-2 (C) upon miR-146a overexpression as compared to scramble control. (B,D) Graph bars showing augmented IFIT-1 (B) and IFIT-2 (D) transcript levels upon silencing of miR-146a by anti-miR-146a. Cy3-labeled scramble sequence was used as negative control. All experiments were repeated thrice and are represented as mean ± SE. The fold change is significant where *denotes P < 0.05, **denotes P < 0.005, and ***denotes P < 0.001.