Table 3.

Effect of ZO-ActFr (50 mg/kg) or standard MCP (30 mg/kg) administered 30 mins before cisplatin challenge, on the level of neurotransmitters and their metabolites (ng/mg tissue wet weight) in the brain areas and intestine in pigeons at t = 3 hr of cisplatin administration (n = 6–8)

Treatment	NA	DOPAC	DA	5HIAA	HVA	5HT
Area postrema
Saline	0.610 ± 0.014	0.382 ± 0.111	0.590 ± 0.146	0.158 ± 0.036	0.913 ± 0.095	0.062 ± 0.034
Cisplatin	1.879 ± 1.622	0.312 ± 0.183	0.080 ± 0.030	0.316 ± 0.101#	0.555 ± 0.188	0.282 ± 0.120
MCP 30 mg	0.023 ± 0.005	0.017 ± 0.006	0.025 ± 0.012	0.005 ± 0.001*	0.121 ± 0.063*	0.023 ± 0.001
ZO-ActFr 50 mg	0.605 ± 0.221	0.080 ± 0.027	1.147 ± 0.615	0.013 ± 0.001***	0.171 ± 0.133	0.006 ± 0.002*
Brain stem
Saline	0.094 ± 0.022	0.060 ± 0.020	0.175 ± 0.078	0.060 ± 0.021	0.060 ± 0.016	0.010 ± 0.003
Cisplatin	0.094 ± 0.024	0.173 ± 0.136	0.030 ± 0.001	0.036 ± 0.004###	0.028 ± 0.003	0.131 ± 0.020###
MCP 30 mg	0.119 ± 0.033	0.027 ± 0.006	0.044 ± 0.012	0.007 ± 0.001***	0.066 ± 0.031	0.019 ± 0.002
ZO-ActFr 50 mg	0.633 ± 0.050	0.015 ± 0.002	0.133 ± 0.077	0.018 ± 0.002***	0.147 ± 0.054	0.037 ± 0.004***
Intestine
Saline	0.194 ± 0.059	0.067 ± 0.020	0.090 ± 0.064	0.076 ± 0.058	0.056 ± 0.025	0.049 ± 0.016
Cisplatin	0.222 ± 0.044	0.015 ± 0.003	0.022 ± 0.005	0.295 ± 0.024###	0.038 ± 0.004	0.665 ± 0.125###
MCP 30 mg	0.138 ± 0.039	0.054 ± 0.025	0.059 ± 0.018	0.097 ± 0.022	0.198 ± 0.102	0.062 ± 0.013
ZO-ActFr 50 mg	0.328 ± 0.036	0.088 ± 0.109	0.005 ± 0.055	0.030 ± 0.013***	0.124 ± 0.066	0.049 ± 0.021***

Values significantly different compared to cisplatin control are indicated as *p < 0.05, ***p < 0.001, while Values significantly different as compared to basal level are indicated as ^#p < 0.05, ^###p < 0.001 (ANOVA followed by Tukey post hoc analysis).