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. 2015 Mar 10;10(3):e0120538. doi: 10.1371/journal.pone.0120538

Fig 5. Actin cytoskeleton and FAK distribution.

Fig 5

MSCs were cultured for 24 h or 7 d with or without drugs, plated on fibronectin-coated dishes overnight, fixed and stained for focal adhesion kinase (FAK) and actin analysis. FAK (green) and actin (magenta) co-localization (white) is demonstrated on merged images. Control cells showed small adhesions at the cell border (A and D) and actin stress fibers (B and E) that showed no variation in the presence of AZA vehicle for 24 h (G-I) or 7 days (J-L). Cells treated with AZA (1 μM) presented large adhesions at the cell rear and smaller adhesions at the cell cortex (M and P), the presence of ventral stress fibers (N) and a tendency of increased presence of dorsal stress fibers (Q). After 24 h or 7 days of DEX treatment, there was an increase in adhesion size throughout the cell body (S and V) and ventral stress fibers (T, W), which was followed by an increase in instable membrane projections at 7 days (X). Bar = 40 μm; representative images of n = 40 cells per condition.