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. 2015 Mar 10;10(3):e0119431. doi: 10.1371/journal.pone.0119431

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Fig 1 — Immunoblotting for PrP^Sc reveals the three characteristic glycoforms. A proteinase K-digested brain homogenate sample from an animal inoculated with H-Type BSE (lane 1) compared to a brain sample from an animal inoculated with classical BSE (lane 2) illustrates the higher molecular weight most noticeable in the BSE- H unglycosylated band. In a brain sample from a negative control animal (lane 3), the proteinase K pre-treatment destroys all antigenicity. Lane 4 contains molecular weight markers.