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. 2015 Mar 10;10(3):e0118861. doi: 10.1371/journal.pone.0118861

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© 2015 Meyer et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — Pull-down assays were performed to determine the effects of phosphorylation on interactions between the ICDs of ACR4 and the CRRs. The autophosphorylation status of each protein was varied for individual experiments, (+) denotes autophosphorylated protein, (-) denotes unphosphorylated protein. In (A), mACR4 (-) and sCRRs (-), (B) mACR4 (+) and sCRRs (+), (C) mACR4 (+) and sCRRs (-), (D) mACR4 (-) and sCRRs (+). In A-D, proteins were separated by 12% SDS-PAGE (top panels) and the presence of interacting proteins was determined by anti-SUMO western blot (lower panels). In the bottom panel of A, anti-MBP western blot demonstrates the presence of mACR4 in each reaction.