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. 2015 Mar 10;10(3):e0118861. doi: 10.1371/journal.pone.0118861

Fig 7. ACR4 ICD interaction with WOX5.

Fig 7

The interaction between sACR4 and mWOX5 was determined by an in vitro pull-down assay. mWOX5 was incubated in the presence of unphosphorylated or phosphorylated sACR4 in parallel experiments. In (A), mWOX5 can specifically bind and pull-down naïve sACR4 (lane 2, arrow). An anti-SUMO western blot confirmed sACR4 binding (lower panel). In controls, no binding is observed to SUMO alone (lane 3) and MBP does not bind to sACR4 (lane 4). In (B) mWOX5 cannot interact with autophosphorylated sACR4. In A and B, 12% SDS-PAGE separation of interacting proteins (top panels) and anti-SUMO western blots of proteins from each reaction (lower panels).