Skip to main content
. 2015 Mar 10;10(3):e0118861. doi: 10.1371/journal.pone.0118861

Fig 8. ACR4 can phosphorylate WOX.

Fig 8

To demonstrate ACR4 phosphorylation of WOX5, sACR4 was incubated with mWOX5 in an in vitro kinase assay. (A) An autoradiogram demonstrating phosphorylation of WOX5. The absence of sACR4 (10 μl load) is shown in lane 1. Phosphorylated mWOX5 (lanes 2–4) was loaded at increasing amounts, 2, 5, and 10 μl of reaction per lane, respectively. A control reaction showing that the MBP tag cannot be phosphorylated by ACR4 (lanes 5–7) was loaded at increasing amounts, 2, 5, and 10 μl of reaction per lane, respectively. (B) The corresponding coomassie stained gel.