Table 1.

Central composite experimental design matrix defining glycerol and (NH ₄ ) ₂ SO ₄ concentrations

Run	Real values		Coded values		Fatty acid production
	X 1	X 2	X 1	X 2	% CDW	g L −1 of culture
1	100	6.23	0	0	45.9	13.9
2	30	1.83	−1	−1	44.3	5.1
3	100	6.23	0	0	45.2	14.3
4	200	6.23	1.41	0	6.0	0.2
5	30	10.63	−1	1	18.2	2.1
6	100	6.23	0	0	44.5	13.2
7	170	10.63	1	1	10.8	0.5
8	170	1.83	1	−1	14.6	0.4
9	0	6.23	−1.41	0	0.0	0.0
10	100	12.45	0	1.41	23.0	6.5
11	100	0	0	−1.41	0.0	0.0

R. opacus MITGM-173 was inoculated in the modified defined media in bioreactors. The data for TAG production as fatty acids represent the maximum values during 10 days of cultivation.

X ₁ glycerol concentration (g L⁻¹), X ₂ (NH₄)₂SO₄ concentration (g L⁻¹).