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. 2015 Mar 11;4(3):e15. doi: 10.1038/emi.2015.15

Figure 4.

Figure 4

High PCV2 viremia made in vivo T-helper cell hypo-responsiveness visible and caused T-cell anergy. (A, B) The TCR was specifically downregulated on CTLs and not on Ths. (A) Three-color flow cytometry with the percentage of total lymphocytes for CTLs and Ths and a TCRlow cell gate (gate: G). TCR expression histogram of PCV2a/b+CsA versus control pigs at day 51 p.t. (B) TCRlow expressing CTLs are significantly more abundant in PCV2a/b+CsA (n=5) (PCV2a/b (n=9), CysA (n=4), control (n=3); pigs per group (n); *P<0.01, Kruskal–Wallis rank sum test). (C–E) IL-2R expression and proliferation of T cells in PMWS versus other pig groups. Two-color flow cytometry analysis of in vitro cultured and ConA- or PMA/IO-stimulated PBMCs. (C, D) IL-2R medium (med.) and high expressing T-cell gates (numbers indicate T-cell percentage of medium and high IL-2R expressers). (D) Statistical comparison of IL-2R medium- and high-expressing T cells compiled from days 41 and 51 p.t. PMWS T cells did not upregulate IL-2R after receptor-mediated (+ConA) in vitro stimuli (PMWS (n=4), PCV2a/b (n=10), CysA (n=4) and control (n=4); data points (n); (*P<0.002, Kruskal–Wallis rank sum test). (E) Statistical comparison of T-cell proliferation compiled from days 41 and 51 p.t. PMWS T cells did not proliferate after receptor-mediated (+ConA) in vitro stimuli (PMWS (n=4), PCV2a/b (n=10), CysA (n=4) and control (n=4); data points (n); (*P<0.005, Kruskal–Wallis rank sum test).