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. 2015 Feb 24;34(1):20. doi: 10.1186/s13046-015-0138-5

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© Chen et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Characterizations of invasive subpopulation of HNSCC cell lines. (A) The invasion assay of A253, RPMI 2650 and their invasive subpopulations. Images show that the cells invaded through the Matrigel coated membrane at a magnification of ×50. (B) Protein expression of EMT related genes and IGFBP-7 in A253, RPMI 2650 and their invasive subpopulations. Expression index was normalized with beta-actin and ratio to A253 or RPMI 2650, respectively. ** indicated p < 0.01; *** indicated p < 0.001.