Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Feb 24;34(1):20. doi: 10.1186/s13046-015-0138-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Chen et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Overexpression of IGFBP-7 in A253-5 cell reverses EMT and suppress cell invasion. (A) Western blot results show up-regulated E-cadherin and down-regulated Vimentin after overexpression IGFBP-7 in A253-5 and reversed by siRNA knockdown. (B) Invasion assay after overexpression of IGFBP-7 in A253-5 cell. Images show that the cells invaded through the Matrigel coated membrane at a magnification of × 100. C: A253-5, Mock: A253-5 transfected with pEGFP-N1, IGFBP7: Transient IGFBP-7 expression A253-5 cells, IGFBP7 + NCsi: Transient IGFBP-7 expression A253-5 cells transfected with negative control siRNA, IGFBP7 + siRNA: Transient IGFBP-7 expression A253-5 cells transfected with siRNA against IGFBP7. * indicated p < 0.05, ** indicated p < 0.01, *** indicated p < 0.001 compared with A253-5 Mock cells. # indicated p < 0.01, ### indicated p < 0.001 compared with IGFBP7 + NCsi.