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. 2015 Mar 10;10(3):e0120395. doi: 10.1371/journal.pone.0120395

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© 2015 Tsai et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — The relative RNA levels of impR in the wild-type, mutants of hfq and micM, and chbIGRov were quantified by real-time RT-PCR. The RNA was prepared using overnight bacterial cultures. The relative RNA levels of MicM in the wild-type, mutants of hfq, impR and hfq/impR, and chbIGRov were also determined in the same way. The expression level for the wild-type cells was set at 1. The data represent the averages of three independent experiments with standard deviations. Significant difference from the wild-type was indicated with the asterisk (*, P<0.05; **, P<0.01; ***, P<0.001 by Student’s t-test analysis). wt, wild-type; hfq, hfq mutant; impR, impR mutant; hfq/impR, hfq and impR double mutant; chbIGRov, chbBC-IGR overexpressing strain; micM, micM mutant.