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. 2015 Mar 10;10(3):e0120395. doi: 10.1371/journal.pone.0120395

Table 1. Bacterial strains and plasmids used in this study.

Strain or plasmid Genotype or relevant phenotype Source or reference
P. mirabilis
wt Wild-type N2; Tcr [2]
hfq wt derivative; hfq-knockout mutant; Kmr [19]
micM wt derivative; micM-knockout mutant; Kmr This study
impR wt derivative; impR-knockout mutant; Cmr This study
hfq/impR wt derivative; hfq/impR-knockout mutant; Kmr Cmr This study
chbIGRov wt containing pGEM-T Easy-chbBC-IGR; chbBC-IGR overexpressing strain; Ampr This study
ImpRov wt containing pGEM-T Easy-impR; impR overexpressing strain; Ampr This study
hfqca hfq mutant containing pGEM-T Easy-hfq; Hfq-complemented strain; Ampr [19]
hfq/impRc hfq/impR mutant containing pGEM-T Easy-impR; hfq/impR mutant complemented with ImpR; Ampr This study
hfqca/impR hfq/impR mutant containing pGEM-T Easy-hfq; hfq/impR mutant complemented with Hfq; Ampr This study
micMc micM mutant containing pGEM-T Easy-micM; MicM-complemented strain; Ampr This study
E. coli
DH5α fhuA2 lac(del)U169 phoA glnV44 Φ80' lacZ(del)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 Invitrogen
S17–1 λ pir λ pir lysogen of S17–1 [thi pro hsdR - hsdM + recA RP4 2-Tc::Mu-Km::Tn7 (Tpr Smr)]; permissive host able to transfer suicide plasmids requiring the Pir protein by conjugation to recipient cells [2]
Plasmids
pGEM-T Easy High-copy TA cloning vector; Ampr Promega
pUT-Km1 Suicide plasmid requiring the Pir protein for replication and containing a mini-Tn5 cassette containing Kmr gene [2]
pACYC184 Low-copy cloning vector, P15A replicon; Cmr Tetr [2]
pGEM-T Easy-chbBC-IGR pGEM-T Easy containing intact chbBC-IGR sequence; Ampr This study
pGEM-T Easy-impR (pImpR) pGEM-T Easy containing intact impR sequence including its ribosome binding site only for overexpression or its promoter for complementation; Ampr This study
pACYC184-chb-xylE chb reporter plasmid, pACYC184 containing intact chb promoter sequence before xylE; Cmr This study
pGEM-T Easy-micM pGEM-T Easy containing intact micM sequence including its promoter; Ampr This study