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. 2015 Mar 10;10(3):e0120395. doi: 10.1371/journal.pone.0120395

Table 2. Primers used in this study.

Primers Sequence (5’ to 3’) Description
micM-upF GAGATCCACACATTCAATC For micM knockout
XbaI-micM-upR TCTAGAAAGCCTCTGGTATCTAAAG
XbaI-micM-downF TCTAGACCTCTTAACGATAGAATAGC For micM knockout
micM-downR AGGCTGAAATGTATTCACC
impR-upF CTGGTGCTGAAGAGGATTTC For impR knockout
XbaI-impR-upR TCTAGATGCCTGGTTTAGCATGTTG
XbaI-impR-downF TCTAGAGTGATCATGCCATTTACG For impR knockout
impR-downR AAATAGACCACACTACGGG
chbBC-IGR-overF GGCCAAGAAGCGGATGTCG For chbBC-IGR overexpression
chbBC-IGR-overR TACCGTCATAAAGGCGGCG
impR-overF AGTCAAAGTCAAAGAGGAAAC For impR overexpression
impR-overR AATACACTTTATCCTTATTG
micMc-F TGATTCTACCATAGAACCATTTCC For micM complementation
micMc-R TGATATCGCCATTGAAAC
chbre-F GCATGCTGTAACGCGAAACAATGC Amplification of chb promoter for reporter assay
chbre-R CTGCAGAACCCTCTCAAATTGGCC
impRrt-F GAAAATGTCTTATGCTGAAG For impR real-time RT-PCR
impRrt-R CGTTAAAGGTAGAGTGGTG
chbBC-IGRrt-F ATATGGGAAAGTGGATGGATTAGG For chbBC-IGR real-time RT-PCR
chbBC-IGRrt-R ACCCTTAAAATGCCGCTAATTG
micMrt-F AAGAGGGCGGAGTGATGA For micM real-time RT-PCR
micMrt-R CGGCCAGTCAAAGAGGAATT
gyrBrt-F GACCCGTACGCTAAACAAC Internal control for real-time RT-PCR
gyrBrt-R AGAAATAACCGCAATCAGG