Table 2. Primers used in this study.
| Primers | Sequence (5’ to 3’) | Description |
|---|---|---|
| micM-upF | GAGATCCACACATTCAATC | For micM knockout |
| XbaI-micM-upR | TCTAGAAAGCCTCTGGTATCTAAAG | |
| XbaI-micM-downF | TCTAGACCTCTTAACGATAGAATAGC | For micM knockout |
| micM-downR | AGGCTGAAATGTATTCACC | |
| impR-upF | CTGGTGCTGAAGAGGATTTC | For impR knockout |
| XbaI-impR-upR | TCTAGATGCCTGGTTTAGCATGTTG | |
| XbaI-impR-downF | TCTAGAGTGATCATGCCATTTACG | For impR knockout |
| impR-downR | AAATAGACCACACTACGGG | |
| chbBC-IGR-overF | GGCCAAGAAGCGGATGTCG | For chbBC-IGR overexpression |
| chbBC-IGR-overR | TACCGTCATAAAGGCGGCG | |
| impR-overF | AGTCAAAGTCAAAGAGGAAAC | For impR overexpression |
| impR-overR | AATACACTTTATCCTTATTG | |
| micMc-F | TGATTCTACCATAGAACCATTTCC | For micM complementation |
| micMc-R | TGATATCGCCATTGAAAC | |
| chbre-F | GCATGCTGTAACGCGAAACAATGC | Amplification of chb promoter for reporter assay |
| chbre-R | CTGCAGAACCCTCTCAAATTGGCC | |
| impRrt-F | GAAAATGTCTTATGCTGAAG | For impR real-time RT-PCR |
| impRrt-R | CGTTAAAGGTAGAGTGGTG | |
| chbBC-IGRrt-F | ATATGGGAAAGTGGATGGATTAGG | For chbBC-IGR real-time RT-PCR |
| chbBC-IGRrt-R | ACCCTTAAAATGCCGCTAATTG | |
| micMrt-F | AAGAGGGCGGAGTGATGA | For micM real-time RT-PCR |
| micMrt-R | CGGCCAGTCAAAGAGGAATT | |
| gyrBrt-F | GACCCGTACGCTAAACAAC | Internal control for real-time RT-PCR |
| gyrBrt-R | AGAAATAACCGCAATCAGG |