FIG 6.

LMB induces nuclear accumulation of Rbs1. Yeast cells were grown to log phase at 30°C (−LMB), and then LMB (100 ng/ml) was added and growth was continued at 30°C for the indicated times before fixation of cells. Localization of Rbs1-Myc was determined by immunofluorescence with anti-Myc antibody in the control wild-type (wt) strain (A), the crm1-T359C mutant (B), and the crm1-T359C rpc128-1007 double mutant (C). Nuclear DNA was stained with DAPI (4′,6′-diamidino-2-phenylindole). (D) The percentage of cells with the given cellular localization of Rbs1 after 1 h of LMB treatment was estimated after inspection of at least 120 single cells.