FIG 2.

USP7 colocalizes with SCML2 and PRC1 components on chromatin. (A) Heat map of normalized ChIP-seq density within a 4-kb window of the top 84 high-confidence USP7 enriched regions (ERs), as identified by model-based analysis of ChIP-Seq. RING1B ChIP-seq data were obtained from GEO series accession number GSE34774. (B) Representative read density tracks at 2 genomic locations demonstrating USP7 occupancy. (C) Cumulative read density across all USP7 ERs depicted in panel A. (D) Immunofluorescence analysis of wild-type HCT116 cells transfected with GFP alone, GFP-SCML2A, or GFP-SCML2AΔRBR and stained with an antibody specific for USP7. The individual channels are shown on the right, and the merged image is shown on the left. (E) Immunoprecipitation of SCML2A or SCML2AΔRBR from nuclear extracts of 293T-REx cells by using the Twin-Strep tag. Pulldown material was analyzed by Western blotting.