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Expression of PRC1 components and histones in HCT116 USP7-KO cells. (A and B) Western blot analysis of the expression of USP7, MDM2, p53, SCML2, and p84 (A) and PRC1 components (B) in whole-cell extracts from wild-type HCT116 (wt) and USP7-KO (USP7−/−) cells. (C) Acid-extracted proteins from HCT116 wild-type and USP7-KO cells were analyzed by Western blotting. Increasing amounts of the extract are loaded for comparison.
