FIG 6.

USP7 inhibition affects PRC1 on chromatin. (A) HCT116 cells were treated with 50 μM P22077, and the expression levels of USP7, SCML2, RING1B, BMI1, CBX2, CBX8, and p53 were analyzed by Western blotting of the nuclear soluble (Nuc) and chromatin (Chr) fractions. (B) Analysis of the expression of histone H2A and the levels of Ub-H2A by Western blotting of the chromatin fraction of samples treated as described above for panel A. (C) Western blot analysis of the chromatin fraction of HCT116 cells after treatment with the USP7 inhibitor HBX19818 for 4 or 8 h at 50 μM. Expression levels of USP7, RING1B, BMI1, and Ub-H2A were analyzed.