FIG 7.

Effects of MG on mTORC2 signaling. (A) Diagram of Akt. PH, the pleckstrin homology domain. (B) Mouse 3T3-L1 adipocytes were treated with 5 mM MG. After being incubated for the prescribed time, the phosphorylation of Thr⁴⁵⁰ in the turn motif (p-Akt TM) and Ser⁴⁷³ in the hydrophobic motif (p-Akt HM) of Akt was determined using the respective specific antibodies. (C) Mouse 3T3-L1 adipocytes were preincubated with 250 nM torin 1 or 20 nM rapamycin (Rap) for 60 min before treatment with 5 mM MG for 60 min.