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. 2015 Feb 25;15:184. doi: 10.1186/s12889-015-1535-6

Table 1.

Details of the primers for HCV amplification

Targeted fragment Name Sequence of oligonucleotide (5’-3’) Position (on H77)
Fragment C/E1 C/E1-F1 ACTGCCTGATAGGGTGCTTGC 288-308
C/E1-R1 GGTGACCAGTTCATCATCATATCC 1301-1324
C/E1-F2 CCTTCCTGGTTGCTCTTTCTCTAT 845-868
C/E1-R2 GTTCATCATCATATCCCAAGCCAT 1293-1315
Fragment NS5b NS5b-F1 CCAATTVVCACTACHATCATGGC 7995-8017
NS5b-R1 TGGRGTGTGTCKDGCTGTTTCC 8789-8810
NS5b-F2 CGTATGATACCCGCTGTTTTGA 8254-8275
NS5b-R2 CCTGGTCATAGCCTCCGTGAA 8616-8636