Table 1.
Gene primer sequences used in RNA-Seq validation
| Name | Gene type | Ensemble gene ID/transcript ID | Primer sequence |
|---|---|---|---|
| RPS16 | Protein-coding | ENSG00000105193 | F: GAAATCCTACCGATAAGCCCA |
| R: TTTCTTGAAACTTTAAAATCCCTCAA | |||
| MMP3 | Protein-coding | ENSG00000149968 | F: ATTCCATGGAGCCAGGCTTTC |
| R: CATTTGGGTCAAACTCCAACTGTG | |||
| COL1A1 a | Protein-coding | ENSG00000108821 | F: GGAGGAGAGTCAGGAAGG |
| R: GCAACACAGTTACACAAGG | |||
| ACAN b | Protein-coding | ENSG00000108821 | F: TCGAGGACAGCGAGGCC |
| R: TCGAGGGTGTAGCGTGTAGAGA | |||
| POU3F4 | Protein-coding | ENSG00000196767 | F: GTTCGCTCGCTCTCTCGTA |
| R: GAAGGGAAGGGAAGGGGAAA | |||
| MYF5 | Protein-coding | ENSG00000111049 | F: TGAACTAATTTTCTGGTCTATATGAC |
| R: TGTACATGTTGTCTTGGTTTGGG | |||
| IGF1 | Protein-coding | ENSG00000017427 | F: CACTATGGACAGATGTAAAAGAAACTA |
| R: ACACACTGGGGACAAGAAATAAA | |||
| MMP16 | Protein-coding | ENSG00000156103 | F: ACCCGTGTAACCCTTTGAGA |
| R: AACCTGAACTTCTTGAACTTGTG | |||
| TGFB3 | Protein-coding | ENSG00000119699 | F: GTAAAGAAAGTGTGGGTTTGGTTA |
| R: AACATCTCAACTTACCATCCCTTT | |||
| EGF | Protein-coding | ENSG00000138798 | F: ACAGGAGGCTTCGGAGTT |
| R: AATCAGGCAATTTACTTACAATCTT | |||
| COL3A1-001 | Protein-coding | ENST00000304636 | F: CAGGTCCCAGCGGTTCT |
| R: CCTTTTGGTCCAGACACTCC | |||
| COL3A1-201 | Protein-coding | ENST00000317840 | F: GGTAGCCCTGGTGAGAGAG |
| R: TGCCAGGAGGTCCAAAGAG | |||
| FGF10-001 | Protein-coding | ENST00000264664 | F: TGCCGTCAAAGCCATTAACA |
| R: CATTTTCCTCTATCCTCTCCTTCA | |||
| CRTAC1-001 | Protein-coding | ENST00000370597 | F:ATCTTCTTCAACAACATTGCCTAC |
| R: GGGTCTCCGTGCTCTCTAC | |||
| IGF1-001 | Protein-coding | ENST00000337514 | F: CAGCAGTCTTCCAACCCAAT |
| R: AAGAGATGCGAGGAGGACAT | |||
| XIST | Lnc | ENSG00000229807 | F: TCCCAGAGAATGCCTAATACTTT |
| R: GTAGAAGAGATACGGAGTAGGAAT | |||
| LINC00957 | Lnc | ENSG00000235314 | F: GAGAGTAAGCAGACCTGGGT |
| R: ACCTTGTCCGAGTTCCATCT | |||
| RP11.308 N19.1 | Lnc | ENSG00000234323 | F: GCCTCTTTCATCACTGCCGA |
| R: TAGCAGCAGTTGGGGTGTTT |