Table 7.
List of top 10 up-/down-regulated isoforms in ageing tendon
| Comparison | Gene | Locus | Log 2 (fold change) | q value |
|---|---|---|---|---|
| Higher in old | PM20D2 | 6:89855768-89875284 | 0.54 | 0.04 |
| ILF3-AS1 | 19:10762537-10764520 | 0.62 | 0.01 | |
| PERP | 6:138411922-138428648 | 0.65 | 0.03 | |
| ODC1 | 2:10580093-10588630 | 0.67 | 0.03 | |
| PRKAA2 | 1:57110994-57181008 | 0.68 | 0.03 | |
| PNRC1 | 6:89790469-89794879 | 0.69 | 0.02 | |
| SYT11 | 1:155829299-155854990 | 0.70 | 0.02 | |
| F13A1 | 6:6144317-6321246 | 0.71 | 0.01 | |
| CITED2 | 6:139693392-139695757 | 0.72 | 0.01 | |
| LDB1 | 10:103867316-103880210 | 0.73 | 0.01 | |
| Lower in old | HLA-DRB5 | 6:32485119-32498064 | −4.72 | 0.01 |
| MYH1 | 17:10286448-10527201 | −4.52 | 0.01 | |
| CRABP2 | 1:156669397-156675608 | −4.44 | 0.03 | |
| CPXM1 | 20:2774714-2781283 | −4.29 | 0.01 | |
| COL3A1 | 2:189839045-189877472 | −4.16 | 0.01 | |
| COL3A1 | 2:189839045-189877472 | −3.87 | 0.01 | |
| ADAM12 | 10:127700949-128077024 | −3.84 | 0.01 | |
| CAPN6 | X:110488330-110513751 | −3.72 | 0.03 | |
| COL1A1 | 17:48260649-48278993 | −3.46 | 0.01 | |
| FAP | 2:163018279-163101661 | −3.37 | 0.01 |
The differentially expressed genes (DEGs) in young versus old tendon were determined by Cuffdiff. The fold change is the ratio of fragments per kilobase of exon per million fragments mapped (FPKM) of those genes in young to FPKM of those genes in old tendon. The significant DEGs (q values of less than 0.05) calculated with a Benjamini-Hochberg correction were ranked on their log2 fold change, and 10 genes with the highest or lowest fold changes are shown in the table.