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HPLC nucleoside analysis of KsgA methylated substrates mutated in the loop of helix 24. (A), (B), and (C), respectively, are chromatograms involving nucleosides derived from 30STag,UM[G791A], 30STag,UM[G791C], and 30STag,UM[G791U] substrates of the 2-hr end point reaction. The top trace in each panel is the measured absorbance at 254 nm of a collection of nucleoside standards including m6A and m62A, while the bottom trace in each panel is measured 3H levels.
