Table 1. Compound-dependent parameters and ion transitions of the analytes and internal standards (ISs) used for the quantification.

Ionization mode	Compound	Molecular íon (m/z)	Ion transition (m/z)	CE a	CEP b	CXP c	DP d	EP e
				(V)	(V)	(V)	(V)	(V)
Positive [M+H]+	Coumarin	147.3	147.3 → 91.0	31	12	4	36	8
			147.3 → 103.1	25	8.4	4	36	8
	7-hydroxycoumarin	163.1	163.1 → 107.1	33	10.8	4	46	4
			163.1 → 77.0	43	11.5	4	46	10
	6-methylcoumarin (IS)	161.0	161.0 → 105.2	27	10	4	61	11.5
			161.0 → 77.1	45	10	4	61	11.5
Negative [M-H]-	o-coumaric acid	162.9	162.9 → 119.0	-18	-10	0.0	-25	-5
			162.9 → 93.1	-30	-10	0.0	-25	-5
	Kaurenoic acid	301.0	301.0	-10	-22	-4	-70	-5
	Isoferulic acid (IS)	192.8	192.8 → 134.2	-20	-10	-4	-25	-6.5
			192.8 → 133.5	-34	-10	-4	-25	-6.5
	Prednisone (IS)	357.0	357.0 → 327.0	-15	-18	-4	-25	-6.5
			357.0 → 299.0	-15	-18	-4	-25	-6.5

Data:

^aCE, collision energy;

^bCEP, collision cell entrance potential;

^cCXP, cell exit potential;

^dDP, declustering potential;

^eEP, entrance potential.