Table 1. Compound-dependent parameters and ion transitions of the analytes and internal standards (ISs) used for the quantification.
Ionization mode | Compound | Molecular íon (m/z) | Ion transition (m/z) | CE a | CEP b | CXP c | DP d | EP e |
---|---|---|---|---|---|---|---|---|
(V) | (V) | (V) | (V) | (V) | ||||
Positive [M+H]+ | Coumarin | 147.3 | 147.3 → 91.0 | 31 | 12 | 4 | 36 | 8 |
147.3 → 103.1 | 25 | 8.4 | 4 | 36 | 8 | |||
7-hydroxycoumarin | 163.1 | 163.1 → 107.1 | 33 | 10.8 | 4 | 46 | 4 | |
163.1 → 77.0 | 43 | 11.5 | 4 | 46 | 10 | |||
6-methylcoumarin (IS) | 161.0 | 161.0 → 105.2 | 27 | 10 | 4 | 61 | 11.5 | |
161.0 → 77.1 | 45 | 10 | 4 | 61 | 11.5 | |||
Negative [M-H]- | o-coumaric acid | 162.9 | 162.9 → 119.0 | -18 | -10 | 0.0 | -25 | -5 |
162.9 → 93.1 | -30 | -10 | 0.0 | -25 | -5 | |||
Kaurenoic acid | 301.0 | 301.0 | -10 | -22 | -4 | -70 | -5 | |
Isoferulic acid (IS) | 192.8 | 192.8 → 134.2 | -20 | -10 | -4 | -25 | -6.5 | |
192.8 → 133.5 | -34 | -10 | -4 | -25 | -6.5 | |||
Prednisone (IS) | 357.0 | 357.0 → 327.0 | -15 | -18 | -4 | -25 | -6.5 | |
357.0 → 299.0 | -15 | -18 | -4 | -25 | -6.5 |
Data:
aCE, collision energy;
bCEP, collision cell entrance potential;
cCXP, cell exit potential;
dDP, declustering potential;
eEP, entrance potential.