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. 2015 Mar 11;5:9012. doi: 10.1038/srep09012

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Huh7.5 control and IFI6 expressing cells were infected with HCVcc and co-localization of CD81 and CLDN1 was determined. Expression of CD81 and CLDN1 on the cell surface of unstimulated control and IFI6 expressing cells is shown (panel A and E, respectively). CD81 (panel B) and CLDN1 (panel C) were detected in fixed mock-transfected control cells. Merged immunofluorescence showing translocation of CD81 and co-localization with CLDN1 in tight junctions of cells is shown (panel D, 6.1% co-localization of CLDN1 with CD81 per field). CD81 (panel F), CLDN1 (panel G), and merged fluorescence displaying localization (panel H, 0.2% co-localization of CLDN1 with CD81 per field) are shown in IFI6 transfected cells. Each panel is representative of three independent experiments.