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. 2015 Mar 11;5:9011. doi: 10.1038/srep09011

Figure 3. Loss of SUMO reduces HSPC generation and proliferation.

Figure 3

(A–C) The vasculature is intact in SUMOs or Ubc9 morphants. WISH assay of flk1 at 30 hpf. (A′–C′) Magnified images of corresponding boxed regions from A to C, respectively. (D–E) The frequency of HSPC generation was reduced in SUMO-deficient embryos. Time-lapse confocal imaging analyses of HSPC generation from Tg (flk1-EGFP) line from 30 to 50 hpf. Representative pictures were captured from Video S1 and Video S2, respectively. Arrowheads indicate the budding cells. DA, dorsal aorta; AV, axial vein. (F) Statistical analyses of the frequency of HSPC generation. Data shown are the mean ± SEM, n = 3, *P < 0.01 by student's t-test. (G–O) The proliferation of HSPC is reduced in SUMOs or Ubc9 morphants. Double immunostaining of cmyb-EGFP (G–I) and BrdU (J–L) in the CHT of Tg (cmyb-EGFP) line at 72 hpf. The bottom panel shows merged images (M–O). (P) Quantification of BrdU and cmyb-EGFP double positive cells in the CHT of Tg (cmyb-EGFP) line at 72 hpf. Data shown are the mean ± SD, n ≥ 3, *P < 0.01 by student's t-test.