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. 2015 Mar 11;5:8997. doi: 10.1038/srep08997

Figure 6. Haploid knockout of PIK3R1 promotes cancer stem cell phenotype through activating CTNNB1.

Figure 6

(a), The WB and RT-PCR analysis of CTNNB1 in 786-mut1 shCtr, 786-mut1 shCTNNB1, A-704-mut2 shCtr, and A-704-mut2 shCTNNB1 cells. (b), The representative photographs of cell spheres formed by 786-mut1 shCtr, 786-mut1 shCTNNB1, A-704-mut2 shCtr, and A-704-mut2 shCTNNB1 cells. Scale bar = 50 μm. (c), 786-mut1 shCTNNB1 and A-704-mut2 shCTNNB1 cells comprised fewer CD44+, CD133+ and CXCR4+ subset, respectively, compared with 786-mut1 shCtr and A-704-mut2 shCtr cells. (d), The tumor formation assays of 786-mut1 shCtrl, 786-mut1 shCTNNB1, A-704-mut2 shCtrl, and A-704-mut2 shCTNNB1 cells. * P < 0.05, ** P < 0.01.