Table 1. Disaccharide composition of CS isolated from estradiol-treated or vehicle-treated MC3T3-E1 cells.
| pmol/mg (mol%)b | ||
|---|---|---|
| Disaccharidesa | Vehicle | Estradiol |
| ΔDi-0S | 22.4 ± 3.7 (5.8) | 17.3 ± 1.8 (3.8) |
| ΔDi-6S | 6.1 ± 0.9 (1.8) | 6.5 ± 1.2 (1.4) |
| ΔDi-4S | 347.0 ± 14.6 (89.8) | 415.9 ± 29.7* (91.6) |
| ΔDi-diSD | N.D.c | N.D. |
| ΔDi-diSE | 10.8 ± 1.7 (2.8) | 14.4 ± 1.9** (3.2) |
| Total | 386.3 ± 15.7 (100) | 454.0 ± 33.9* (100) |
aAbbreviations: ΔDi-0S, ΔHexA-GalNAc; ΔDi-6S, ΔHexA-GalNAc(6-O-sulfate); ΔDi-4S, ΔHexA-GalNAc(4-O-sulfate); ΔDi-diSD, ΔHexA(2-O-sulfate)-GalNAc(6-O-sulfate); ΔDi-diSE, ΔHexA-GalNAc(4,6-O-disulfate). ΔHexA represent an unsaturated hexuronic acid that was generated via catalysis by bacterial CS-degrading enzymes, including chondroitinase ABC.
bThe values are expressed as pmol of disaccharide per mg of dried homogenate and represent the mean ± s.d. (n = 4). The values in parentheses represent molar ratio of the disaccharides.
cN.D., not detected.
*, P < 0.05;
**, P < 0.01.