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. 2015 Mar 11;5:9004. doi: 10.1038/srep09004

Figure 3. The fluorescence intracellular localization of Superior LysoProbe (IV) (1 μM) was examined in an additional three cancer cell lines: gastric cancer-derived HGC-27 cells, human colon cancer-derived CW-2 cells, and human breast cancer MCF-7 cell lines.

Figure 3

The intracellular distribution of Superior LysoProbe (IV) (1 μM) compared to LysoTracker Green (2 μM) in human colon cancer-derived CW-2 cells, gastric cancer-derived HGC-27 cells, and human breast cancer MCF-7 cell lines is shown. Superior LysoProbe (IV) (1 μM) was incubated with cells in non-FBS DMEM media for 15 min., and then counterstained with LysoTracker (2 μM, weak green fluorescence), Hoechst 33342 (1 μg/mL, blue). The cells were imaged on a confocal laser-scanning fluorescent microscope (Olympus) using a 60 × oil immersion objective lens.