Figure 9. Confocal laser-scanning fluorescent images of RBE cells treated with lobaplatin (5 μg/mL) with and without CQ (20 μM) for 24 h.

GFP-LC3 (green fluorescence) transfected RBE cells were labeled with Superior LysoProbe (IV) (1 μM, red fluorescence), and Hoechst 33342 (1 μg/ml, blue fluorescence). Cells were imaged on an inverted laser scanning fluorescent microscope (Olympus) using a 60 × oil immersion objective lens.