Table 1.
Oligonucleotide primers and PCR cycling profiles used in the study
| Fragment | Primer | Position1 | Sequence | Denaturation | Annealing | Extension | Size2 |
| A | 455(+) | 455-474 | 5’-CAAGGTATGTTGCCCGTTTG-3 | 1 345 | |||
| 1 800(–) | 1 800-1 773 | 5’-AGACCAATTTATGCCTACAGCCTCCTA-3’ | 94 °C 30 s | 62 °C 30 s | 72 °C 90 s | ||
| B | 1 687(+) | 1 687-1 708 | 5’-CGACCGACCTTGAGGCATAC-3’ | ||||
| 685(-) | 704-685 | 5’-CGAACCACTGAACAAATGGC-3’ | 94 °C 30 s | 63 °C 30 s | 72 °C 30 S | 2 198 |
(+) sense (–) anti-sense.
1
Denotes the nucleotide position of HBV adw genome (GenBank accession no. V00866) where the EcoRI cleavage site is position 12. Size of the amplicons in base pairs.