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. Author manuscript; available in PMC: 2015 Aug 26.
Published in final edited form as: Nature. 2015 Feb 26;518(7540):560–564. doi: 10.1038/nature14234

Figure 1. m6A alters RNA structure to enhance hnRNP C binding.

Figure 1

a, m6A increases binding with nuclear proteins. b, RNA pull down showing hnRNP C preferably binds methylated RNA. n = 4, ± s.d., biological replicates. c, Filter binding showing m6A increases hnRNP C1 binding with respective apparent dissociation constant (Kd) indicated at lower right; n = 3, ± s.d., technical replicates. d, RNA structural probing showing m6A disrupts local RNA structure highlighted in yellow. Ctrl, no nuclease added; V1, RNase V1 digestion; S1, nuclease S1 digestion; T1, RNase T1 digestion; G-L, G-ladder; AH, alkaline hydrolysis. The orange bars mark the structurally altered RNA regions in the presence of m6A (red dot). e, RNA pull down with mutated oligos. n = 3, ± s.d., technical replicates. f, Illustration of the m6A-switch model.