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. Author manuscript; available in PMC: 2015 Mar 11.
Published in final edited form as: Differentiation. 2014 Mar 6;87(0):134–146. doi: 10.1016/j.diff.2014.02.005

Fig. 4.

Fig. 4

The absence of Meckelin and Jouberin impairs mESCs response to retinoic acid. mESCs were transfected with siMeckelin and siJbn in the absence of serum and antibiotics and in the presence of LIF. After 12 h, mESCs were re-fed with fresh LIF-containing medium. After additional 24 h, cells were shifted to LIF-deprived medium and treated with RA or control solvent for 5 days. (A) Upper panel. Immunofluorescence showing the reduction in Jouberin and Meckelin expression. Lower panel. Immunofluorescence showing the dramatic decrement of β-tubulin III protein in siMeckelin and siJbn mESCs. (B) Western blot analyses showing both the down-regulation of Meckelin and Jbn in siRNA-transfected cells, the modulation of shh and wnt pathways and the effect on the expression of the transcription factors Gbx2 and En2. These results are representative of three independent experiments. Uncropped western blots are shown in Supplementary Fig. S7A and B.