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. Author manuscript; available in PMC: 2015 Mar 11.
Published in final edited form as: J Crit Care. 2013 Oct 29;29(1):24–30. doi: 10.1016/j.jcrc.2013.10.010

Figure 3. Fibrinogen consumption in whole blood.

Figure 3

Whole blood sera were diluted with 1x SDS-PAGE sample buffer, and run out on 4–20% SDS-PAGE under non-reducing conditions followed by Western blotting probing with an anti-Fibrinogen-2a mouse monoclonal antibody. Densitometry was performed on the blots for the 37°C (●), 32°C (■) and 27°C (▲) time courses (N=3). Data are shown as the mean±SEM of the normalized percent activation.