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Exogenous ATP affects twitching motility of P. aeruginosa. Time-lapse phase-contrast microscopy of P. aeruginosa PAK interstitial biofilm expansion on solidified nutrient media-coated microscope slide containing 0 mM, 2.5 mM, 5 mM or 7.5 mM ATP and a coverslip at 37°C. Scalebar is 10 μm. Time (min:s) indicated in top right.
