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Extracellular ATP gradients affect twitching motility of P. aeruginosa. Time-lapse phase-contrast microscopy of P. aeruginosa PAK interstitial biofilm expansion in the presence of an ATP- or H2O-saturated disc after 4 h of incubation at 37°C. Scalebar is 10 μm. Time (min:s) indicated in top right.
