Table 1.
Sequences for primers
| Gene | Gene ID | Primers | Sequences |
|---|---|---|---|
| Renin | NM_012642 | Forward | 5′-CAGGAACGATGACCTGTGCAT-3′ |
| Reverse | 5′- CAGTGGGTGGTGGGATGTC-3′ | ||
| AT1-R | NM_030985 | Forward | 5′-CTCAAGCCTGTCTACGAAAATGAG -3′ |
| Reverse | 5′-GTGAATGGTCCTTTGGTCGT -3′ | ||
| AT2-R | NM_012494 | Forward | 5′-TGCTGTTGTGTTGGCATTCA-3′ |
| Reverse | 5′-ATCCAAGAAGGTCAGAACATGGA-3′ | ||
| ACE-1 | NM_012544 | Forward | 5′-TTTGCTACACAAATGGCACTTGT-3′ |
| Reverse | 5′-CGGGACGTGGCCATTATATT-3′ | ||
| ACE-2 | NM-001012006 | Forward | 5′-TTGAACCAGGATTGGACGAAA-3′ |
| Reverse | 5′-GCCCAGAGCCTACGATTGTAGT-3′ | ||
| TNF-α | NM_013693 | Forward | 5′-GCATGATCCGCGACGTGGAA-3′ |
| Reverse | 5′-AGATCCATGCCGTTGGCCAG-3′ | ||
| IL-1β | NM_031512 | Forward | 5′-TGA TGT TCC CAT TAG ACA GC-3′ |
| Reverse | 5′-GAG GTG CTG ATG TAC CAG TT-3′ | ||
| IL-10 | NM_012854 | Forward | 5′-GTTGCCAAGCCTTGTCAGAAA-3′ |
| Reverse | 5′-TTTCTGGGCCATGGTTCTCT-3′ | ||
| GAPDH | NM_017008 | Forward | 5′-GCCAAAAGGGTCATCATCTC-3′ |
| Reverse | 5′-GGCCATCCACAGTCTTCT-3′ |
AT, angiotensin receptor type; ACE, angiotensin-converting enzyme; IL, interleukin; TNF, tumor necrosis factor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.