Anthropometric outcomes |
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Height |
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Portable stadiometer (model TZG, China) |
Measured to the nearest mm without shoes. |
Weight |
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Lever type weight scale (model RGT-140,China) |
Measured to the nearest 0.1 kg without shoes and in light clothing |
Waist circumference |
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Steel tape |
Measured to the nearest mm and located at the 1 cm above umbilicus |
Hip circumference |
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Steel tape |
Measured to the nearest mm and located at maximal protrusion of the buttocks |
Blood pressure |
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Mercury sphygmomanometer, (model XJ1ID, China) and TZ-1 stethophone |
Measured from the right arm using a validated mercury sphygmomanometer. The mid upper arm circumference determined cuff size. The cuff was placed approximately 2 cm above the crease of the elbow. The child was seated comfortably for at least 5 minutes prior to the first reading. Blood pressure was measured two times, with one minute between each measurement. Children were asked to remain quiet and to sit still while each reading was being taken. Systolic blood pressure and diastolic blood pressure were recorded. |
Behavioral outcomes |
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Children Questionnaire; Parent Questionnaire; School Questionnaire |
Children under the third grade, both children and parent questionnaires would be filled by parents; Children above the fourth grade, would fill in children questionnaire by themselves in class, instructed by class teacher, and parent questionnaire would be filled by their parents; all the school questionnaire would by filled by school principal or school doctor. |
Blood chemical outcomes |
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After a 12-h overnight fast, 5 ml venous blood samples were taken from the antecubital vein and collected into EDTA vacuum tubes. Samples were centrifuged at 3000r, aliquoted and stored at −80°C. All biochemical analyses on blood were carried out at a validated biomedical analyses laboratory. |
Fasting glucose |
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Glucose oxidase method |
Triglycerides |
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Enzymatic methods |
Cholesterol |
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Enzymatic methods |
HDL-C |
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Clearance method |
LDL-C |
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Clearance method |
Physical fitness outcomes |
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Standardized sport equipment |
Measured by physical education teachers under the instruction of the project members according to uniform measurement standards |