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. 2015 Mar 11;9:62. doi: 10.3389/fnbeh.2015.00062

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Copyright © 2015 Speranza, Giuliano, Volpicelli, De Stefano, Lombardi, Chambery, Lacivita, Leopoldo, Bellenchi, di Porzio, Crispino and Perrone-Capano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stimulation of 5-HT7R activates mTORC1 signaling. Cortical neurons were treated for 2 h with the 5-HT7R selective agonist LP-211 (LP, 100 nM), or the mTOR inhibitor torin 1 (TR1, 250 nM), alone or in combination. (A) The bars show the level of p70S6K phosphorylation at Thr389 (means ± SEM, n = 6), expressed as percentage of values measured in the corresponding vehicle-treated cultures (CTRL, set to 100%). The level of p70S6K phosphorylation was measured by Western blot as intensity of p70S6K phosphorylated at Thr389 (p-p70S6K) normalized with that of total (phosphorylated and unphosphorylated) p70S6K in the same samples. The inset displays representative blots probed with antibodies against p-p70S6K and p70S6K; the molecular weights (kDa) are shown on the right. (B) The bars show the level of Akt phosphorylation at Ser473 (means± SEM, n = 3), expressed as percentage of values measured in the corresponding vehicle-treated cultures (CTRL, set to 100%). The level of Akt phosphorylation was measured by Western blot as intensity of Akt phosphorylated at Ser473 (p-Akt) normalized with that of total Akt (Akt) in the same samples. The inset displays representative blots probed with antibodies against p-Akt and Akt; the molecular weights (kDa) are shown on the right. Asterisk (*): value significantly different from CTRL by One Way ANOVA followed by Dunnett post-hoc test (p < 0.05).