Table 1.

Alpha-ketoglutarate dependent enzymes as mediators of synergistic function.

	Enzyme	Km (mM)
		α‡	Iron (II)	2-oxoglutarate	Dioxygen	Substrates	References
A	Prolyl 4-hydroxylase (HP4H)	0.11	0.0031	0.03	0.186	0.053	De Jong and Kemp, 1984; Hirsila et al., 2003; Kukkola et al., 2003; Myllyharju, 2003, 2008
B	Prolyl 4-hydroxylase (CP4H)	0.12	0.0031	0.021	0.04	0.311	Kivirikko and Myllyla, 1982; Kivirikko et al., 1989; Helaakoski et al., 1995; Lamberg et al., 1995; Annunen et al., 1997, 1998, 1999; Hieta et al., 2003; Kukkola et al., 2003; Myllyharju, 2003, 2008; Kersteen et al., 2004
C	Prolyl 3-hydroxylase (cis)	0.10		0.08		0.3975	Mori et al., 1997; Shibasaki et al., 1999, 2000
D	Prolyl 3-hydroxylase (trans)	0.10	0.0005	0.08	0.03	0.165	Tryggvason et al., 1979; Tiainen et al., 2008
E	Asparagine hydroxylase	0.17	0.0005	0.019	0.09	0.01	Hewitson et al., 2002; Koivunen et al., 2004; Flagg et al., 2012
*Subcase 1	A + E		F = (3.0266)X1 + (4.3943)X2 + 0.9469				X1:= 4-hydroxyproline
							X2:= hydroxyasparagine
*Subcase 2	(A + B) + E		F = (7.54375)X1 + (4.3943)X2 + 0.58821				X1:= hydroxyproline
							X2:= hydroxyasparagine
*Subcase 3	(A + B + C + D) + E		F = (1.6997)X1 + (4.3943)X2 + 0.6779				X1:= hydroxyproline
							X2:= hydroxyasparagine

^*Coefficient details (S3): Subcase 1: X1:: se = 0.4515, p = 1.3E-09, df = 99; Subcase 2: X1:: se = 0.09281, p < 2.00E-16, df = 99; Subcase 3: X1:: se = 0.1825, p = 3.7E-15, df = 99; Subcases 1, 2, 3: X2:: se = 0.4954, p = 3.41E-14, df = 99.

^‡See Table T2C in Data sheet 2.