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. 2015 Mar 11;9:90. doi: 10.3389/fncel.2015.00090

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Copyright © 2015 Phillips-Farfán, Rubio Osornio, Custodio Ramírez, Paz Tres and Carvajal Aguilera.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Brief diagram of the mechanistic target of rapamycin (mTOR) pathway with analyzed proteins/genes (black arrows, activation; red lines, inhibition; AMPK, adenosine monophosphate-activated protein kinase; PKB, protein kinase B; TSC1, tuberous sclerosis 1; TSC2, tuberous sclerosis 2; S6K, ribosomal protein S6 kinase; S6, ribosomal protein S6). (B) Representative blots showing both phosphorylated (p) and total AMPK, PKB and S6 in the neocortex (Cx) and hippocampus (Hp) of rats fed ad libitum (AL) or subjected to 15% caloric restriction (CR). The molecular weight (MW) is shown in kilodaltons (kDa) to the right of each protein pair.