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. Author manuscript; available in PMC: 2015 Mar 11.
Published in final edited form as: Protein Expr Purif. 2010 Apr 22;74(1):49–59. doi: 10.1016/j.pep.2010.04.006

Fig. 2.

Fig. 2

Concentration dependence of the uptake of [3H]-d-aspartate into HEK 293T cells expressing either 6×HIS hEAAT2 or native hEAAT2. The transport rates were determined as described in Materials and methods and have been corrected for non-specific uptake and leakage (4 °C). Data are reported as means ± SEM from a minimum of 4 determinations. Kinetic constants (reported in the text) were determined by non-linear curve fitting (KaleidaGraph 3.6).