Table 1.

Tryptic peptides of 6 × HIS hEAAT2 observed by MALDI-TOF MS.

Peptide position	m/z observed (Da)	In-gel	In-solution	Missed cleavages	Modification
001–025	2610.48 mi	X
001–052	5562.39 avg		X	3
026–047	2405.68 mi		X	1
053–064	1366.42 mi	X
053–071	2238.16 avg			2
076–122	4931.46 avg			2
101–122	2438.49 avg	X			2Met-ox
101–125	2811.03 avg		X	1
101–150	5317.59 avg		X	3	1Met-ox
123–146	2558.32 avg		X	1
155–183	3056.42 avg		X
184–192	969.71 avg		X	2
194–208	1706.88 mi	X	X
209–228	2378.10 mi	X			1CAM
230–262	3449.10 avg	X
263–272	1210.68 avg		X	2
315–334	2372.41 avg	X			2CAM, 2Met-ox
315–376	6940.20 avg			3	2Met-ox
347–408	6765.80 avg			2
409–419	1346.85 avg	X			1CAM
423–511	9013.93 avg		X
423–513	9300.75 avg			1
514–533	2050.03 mi	X	X
534–544	1300.60 mi	X
534–553	2400.63 avg	X	X	1	1Met-ox
554–562	1100.18 mi	X
563–606	4835.07 avg		X	3
566–586	2424.22 mi	X			2CAM
595–607	1592.73 mi	X		1	1CAM

mi = observed as a monoisotopic mass; avg = observed as an average mass; CAM = carbamidomethyl cysteine.