Table 3.
Differentially expressed proteins in fruit flesh of Temple (Te) versus Murcott (Mu) mandarin hybrid fruit
| Accession | Name | Species | iTRAQ ratio fold change | |||||
|---|---|---|---|---|---|---|---|---|
| Stage 1 | Stage 2 | Stage 3 | ||||||
| Te/Mu | P value | Te/Mu | P value | Te/Mu | P value | |||
| gi|11596186 | cystatin-like protein | Citrus x paradisi | 4.041 | 0.036 | 0.647 | 0.002 | ||
| gi|118061963 | extracellular solute-binding protein, family 5 | Roseiflexus castenholzii DSM 13941 | 0.483 | 0.047 | ||||
| gi|119367477 | putative H-type thioredoxin | Citrus cv. Shiranuhi | 10.782 | 0.001 | 0.404 | 0.001 | ||
| gi|119367479 | putative cyclophilin | Citrus cv. Shiranuhi | 0.588 | 0.037 | 2.347 | 0.002 | ||
| gi|121485004 | cytosolic phosphoglycerate kinase | Helianthus annuus | 5.535 | 0.002 | ||||
| gi|124360080 | Galactose mutarotase-like | Medicago truncatula | 1.724 | 0.003 | ||||
| gi|125546170 | hypothetical protein OsI_14032 | Oryza sativa Indica Group | 0.561 | 0.014 | ||||
| gi|14031067 | dehydrin COR15 | Citrus x paradisi | 2.806 | 0.000 | ||||
| gi|147809484 | hypothetical protein | Vitis vinifera | 0.608 | 0.022 | 0.696 | 0.065 | ||
| gi|147836508 | hypothetical protein | Vitis vinifera | 1.630 | 0.024 | ||||
| gi|147853192 | hypothetical protein | Vitis vinifera | 1.803 | 0.018 | ||||
| gi|15219028 | 26.5 kDa class I small heat shock protein-like | Arabidopsis thaliana | 0.491 | 0.008 | ||||
| gi|15235730 | phosphoenolpyruvate carboxykinase (ATP), putative/PEP carboxykinase, putative/PEPCK, putative | Arabidopsis thaliana | 1.899 | 0.034 | ||||
| gi|159471948 | U2 snRNP auxiliary factor, large subunit | Chlamydomonas reinhardtii | 0.255 | 0.044 | ||||
| gi|166850556 | CTRSFT1-like protein | Poncirus trifoliata | 3.261 | 0.011 | 0.237 | 0.005 | ||
| gi|169160465 | phospholipase D alpha | Citrus sinensis | 4.060 | 0.000 | 0.240 | 0.000 | 0.573 | 0.000 |
| gi|17225598 | pyruvate decarboxylase | Fragaria x ananassa | 0.286 | 0.012 | ||||
| gi|183579873 | chitinase | Citrus unshiu | 1.534 | 0.012 | ||||
| gi|192912988 | 40S ribosomal protein S4 | Elaeis guineensis | 1.601 | 0.049 | ||||
| gi|218202932 | 14-3-3 protein | Dimocarpus longan | 0.227 | 0.016 | ||||
| gi|221327587 | ascorbate peroxidase | Citrus maxima | 4.863 | 0.000 | 0.180 | 0.049 | ||
| gi|2213425 | hypothetical protein | Citrus x paradisi | 0.627 | 0.000 | 0.524 | 0.001 | ||
| gi|223949137 | unknown | Zea mays | 5.116 | 0.003 | ||||
| gi|224069008 | predicted protein | Populus trichocarpa | 6.992 | 0.001 | ||||
| gi|224099429 | predicted protein | Populus trichocarpa | 0.587 | 0.014 | 0.316 | 0.002 | ||
| gi|224109966 | predicted protein | Populus trichocarpa | 0.476 | 0.040 | ||||
| gi|224127346 | predicted protein | Populus trichocarpa | 0.156 | 0.007 | 0.641 | 0.043 | ||
| gi|224128794 | predicted protein | Populus trichocarpa | 0.298 | 0.007 | 0.382 | 0.022 | ||
| gi|224135985 | predicted protein | Populus trichocarpa | 0.248 | 0.006 | 0.366 | 0.021 | ||
| gi|225424861 | PREDICTED: hypothetical protein isoform 2 | Vitis vinifera | 0.536 | 0.040 | ||||
| gi|225425914 | PREDICTED: hypothetical protein | Vitis vinifera | 0.429 | 0.002 | 0.425 | 0.010 | ||
| gi|225439785 | PREDICTED: hypothetical protein | Vitis vinifera | 0.441 | 0.007 | 0.658 | 0.023 | ||
| gi|225441981 | PREDICTED: hypothetical protein | Vitis vinifera | 0.304 | 0.002 | 0.568 | 0.007 | ||
| gi|225442225 | PREDICTED: hypothetical protein | Vitis vinifera | 9.896 | 0.015 | 0.576 | 0.010 | 0.571 | 0.002 |
| gi|225451968 | PREDICTED: similar to mangrin | Vitis vinifera | 4.507 | 0.040 | 0.263 | 0.095 | ||
| gi|231586 | ATP synthase subunit beta | Hevea brasiliensis | 0.134 | 0.004 | 0.555 | 0.007 | ||
| gi|242199340 | UDP-glucosyltransferase family 1 protein | Citrus sinensis | 7.535 | 0.002 | 0.394 | 0.008 | 0.539 | 0.030 |
| gi|255539613 | phosphoglucomutase, putative | Ricinus communis | 0.142 | 0.020 | ||||
| gi|255543156 | conserved hypothetical protein | Ricinus communis | 7.967 | 0.000 | ||||
| gi|255544686 | eukaryotic translation elongation factor, putative | Ricinus communis | 0.424 | 0.006 | 0.323 | 0.008 | ||
| gi|255550111 | heat-shock protein, putative | Ricinus communis | 3.788 | 0.043 | ||||
| gi|255551036 | aspartate aminotransferase, putative | Ricinus communis | 0.599 | 0.037 | ||||
| gi|255561582 | Patellin-3, putative | Ricinus communis | 0.588 | 0.017 | ||||
| gi|255571742 | peptidase, putative | Ricinus communis | 0.275 | 0.004 | ||||
| gi|255586766 | monodehydroascorbate reductase, putative | Ricinus communis | 0.429 | 0.003 | 0.493 | 0.001 | ||
| gi|255641409 | unknown | Glycine max | 0.645 | 0.021 | ||||
| gi|255642211 | unknown | Glycine max | 0.521 | 0.011 | 0.121 | 0.001 | ||
| gi|255644696 | unknown | Glycine max | 5.914 | 0.002 | ||||
| gi|257659867 | unnamed protein product | Linum usitatissimum | 0.329 | 0.235 | 0.368 | 0.047 | ||
| gi|257675725 | unnamed protein product | Zea mays | 3.832 | 0.019 | ||||
| gi|257690969 | unnamed protein product | Citrus sinensis | 0.384 | 0.002 | ||||
| gi|257712573 | unnamed protein product | Brassica napus | 9.086 | 0.011 | 0.664 | 0.006 | ||
| gi|257720002 | unnamed protein product | Glycine max | 0.551 | 0.001 | 0.387 | 0.007 | ||
| gi|257726687 | unnamed protein product | Zea mays | 1.650 | 0.035 | 0.387 | 0.001 | ||
| gi|27462762 | malate dehydrogenase | Lupinus albus | 0.305 | 0.003 | ||||
| gi|29124973 | unknown | Populus tremuloides | 2.039 | 0.031 | ||||
| gi|33316389 | valencene synthase | Citrus sinensis | 25.730 | 0.022 | ||||
| gi|33325127 | eukaryotic translation initiation factor 5A isoform VI | Hevea brasiliensis | 1.914 | 0.039 | ||||
| gi|33340236 | copper/zinc superoxide dismutase | Citrus limon | 3.706 | 0.001 | 0.638 | 0.004 | ||
| gi|37524017 | COR15 | Citrus clementina x Citrus reticulata | 10.311 | 0.006 | 2.382 | 0.010 | ||
| gi|3790102 | pyrophosphate-dependent phosphofructokinase alpha subunit | Citrus x paradisi | 1.724 | 0.025 | 0.554 | 0.011 | ||
| gi|40646744 | mitochondrial citrate synthase precursor | Citrus junos | 0.201 | 0.032 | 0.553 | 0.018 | ||
| gi|4580920 | vacuole-associated annexin VCaB42 | Nicotiana tabacum | 0.209 | 0.046 | 0.330 | 0.007 | ||
| gi|4704605 | glycine-rich RNA-binding protein | Picea glauca | 4.452 | 0.009 | ||||
| gi|530207 | heat shock protein | Glycine max | 4.177 | 0.045 | ||||
| gi|544437 | Probable phospholipid hydroperoxide glutathione peroxidase | Citrus sinensis | 3.140 | 0.039 | ||||
| gi|5764653 | NADP-isocitrate dehydrogenase | Citrus limon | 0.430 | 0.006 | 0.437 | 0.003 | ||
| gi|6094476 | Thiazole biosynthetic enzyme | Citrus sinensis | 0.228 | 0.007 | ||||
| gi|6166140 | Elongation factor 1-delta 1 | Oryza sativa Japonica Group | 7.427 | 0.045 | 0.654 | 0.028 | ||
| gi|6225526 | Isopentenyl-diphosphate Delta-isomerase I | Clarkia breweri | 0.562 | 0.033 | ||||
| gi|624674 | heat shock protein | Citrus maxima | ||||||
| gi|624676 | citrate synthase precursor | Citrus maxima | 2.731 | 0.020 | ||||
| gi|62900641 | Plastid-lipid-associated protein | Citrus unshiu | 6.082 | 0.002 | 0.289 | 0.000 | 0.662 | 0.022 |
| gi|63333659 | beta-1,3-glucanase class III | Citrus clementina x Citrus reticulata | 0.493 | 0.141 | 2.712 | 0.000 | ||
| gi|6518112 | H + −ATPase catalytic subunit | Citrus unshiu | 4.754 | 0.017 | 0.598 | 0.007 | ||
| gi|6682841 | sucrose synthase | Citrus unshiu | 3.194 | 0.025 | 0.632 | 0.009 | ||
| gi|6682843 | sucrose synthase | Citrus unshiu | 0.144 | 0.008 | 0.575 | 0.024 | ||
| gi|7024451 | glycine-rich RNA-binding protein | Citrus unshiu | 1.886 | 0.531 | ||||
| gi|70609690 | glutamate decarboxylase | Citrus sinensis | 3.588 | 0.025 | 0.643 | 0.043 | ||
| gi|7269241 | UDPglucose 4-epimerase-like protein | Arabidopsis thaliana | 0.424 | 0.011 | 0.158 | 0.004 | ||
| gi|74486744 | translation elongation factor 1A-9 | Gossypium hirsutum | 4.923 | 0.008 | ||||
| gi|76573375 | triosphosphate isomerase-like protein | Solanum tuberosum | 0.311 | 0.000 | ||||
| gi|77417715 | SOD | Citrus maxima | 0.638 | 0.017 | 0.118 | 0.010 | 0.322 | 0.013 |
| gi|77540216 | triosephosphate isomerase | Glycine max | 0.514 | 0.022 | ||||
| gi|77744899 | temperature-induced lipocalin | Citrus sinensis | 4.028 | 0.018 | 0.548 | 0.016 | ||
| gi|82623427 | glyceraldehyde 3-phosphate dehydrogenase-like | Solanum tuberosum | 0.661 | 0.297 | ||||
| gi|862480 | valosin-containing protein | Glycine max | 1.510 | 0.029 | 0.374 | 0.010 | ||
| gi|870794 | polyubiquitin | Arabidopsis thaliana | 4.534 | 0.005 | ||||
| gi|90820120 | UDP-glucose pyrophosphorylase | Cucumis melo | 7.835 | 0.028 | ||||
| gi|9082317 | actin | Helianthus annuus | 3.959 | 0.051 | 0.527 | 0.001 | ||
| gi|9280626 | UDP-glucose pyrophosphorylase | Astragalus membranaceus | 9.821 | 0.002 | 1.626 | 0.022 | ||
| gi|9757974 | polyubiquitin | Arabidopsis thaliana | 0.585 | 0.011 | ||||
The P value was selected from the most significant one among three biological replications. Additional file 1: Table S2 has the result from all three biological replications. Stage 1 was on December 22, 2008, Stage 2 was on January 30, 2009, and Stage 3 was on March 11, 2009.