FIG. 7.

Correlation between speed and distance to the nearest radial process. (A) The speed (solid line) of a control cell (C57) and the distance (—○—) to the nearest radial glial process plotted as a function of time. Arrows show periods of stalling. (B) A cell treated with 1 μM pyr3. (C, D) Statistics for experiments similar to (A, B). Cells tracked had to move with a speed above 60 μm/h at least twice or more during a period 10 h or more. In addition, each cell needed to have at least 500 μm free space to move without other neurospheres. (C) The distance to the nearest radial process as a function of time for control cells (N=23, •), pyr3-treated cells (N=18, o), 3/6 DKO cells (N=20, ▴), 1/4/5 TKO cells (N=17, ▵), and MPEP-treated cells (N=21, ▪). (D) The distance to the nearest radial glial process during the initial periods of stalling (speed<40 μm/h). Average±SD. (E) A three-dimensional bar graph plotting frequency count of stalling cells as percentage of total time points (Z axis) versus distance from radial glial processes (Y axis) in 20 μm increments, for controls, pyr3, MPEP, 3/6 DKO, and 1/4/5 TKO cells (X axis). The stalling control cells accumulated at a distance of 20 μm from the radial processes, while the distribution of frequency count for pyr3-treated and 3/6 DKO cells was broad over a wide range of longer distances. The 1/4/5 TKO cells showed a distribution more similar to the control cells. (F) An area plot diagram of the same data in (E) of WT versus pyr3 showing the distribution profile of the distance increments of the stalling cells. ns, not significant; *P<0.05; **P<0.01; ***P<0.001.