Table 1.
Primers for Quantitative Real-Time Polymerase Chain Reaction
| Target | Forward primers (5′–3′) | Reverse primers (5′–3′) | Product size (bp) |
|---|---|---|---|
| Trpc1 | TTGCTGGCGTGCGACAAGGG | GTGCATCTGCGGACTGACAACCG | 187 |
| Trpc3 | GGAGAGCGATCTGAGCGAAGT | GGGAGCCATTTGTCTCTAGCA | 75 |
| Trpc4 | TGTAGGCGATGCGCTGCTTCA | GAATGGGAGGCACCTGCTTCTCTC | 107 |
| Trpc5 | GCCATCCGCAAGGAGGTGGTAG | CATCATCAGCGTGGGAACCTGCT | 90 |
| Trpc6 | ACTACATTGGCGCAAAACAGAA | AGAAAGACCAAAGATAGCCCAGAA | 86 |
| Trpc7 | TCCATAGGCCTCCCTTTTCTC | TCCTCAGGGTTTGTCCTAGCTT | 73 |
| β-actin | GTGGATCAGCAAGCAGGAGT | GAAAGGGTGTAAAACGCAGC | 99 |
| GAPDH | AACGACCCCTTCATTGAC | TCCACGACATACTCAGCAC | 191 |
| 18S rRNA | AAACGGCTACCACATCCAAG | GAAAGCTCCGGGACATTAAC | 112 |
TRPC, canonical transient receptor potential channels.