FIG. 2.

Transduction of unstimulated and stimulated human CD105⁺/CD34⁺ cells by CD105-LV^GFP. Unstimulated or stimulated CD34⁺ cells isolated from G-CSF mobilized peripheral blood were transduced by CD105-LV^GFP at MOI of 1. Seventy-two hours after transduction the cells were analyzed by flow cytometry for CD105 and GFP expression. (A) Representative FACS plots of GFP⁺ cells transduced with or without previous stimulation of cells. (B) Mean±SD of GFP⁺ cells of seven independent experiments. GFP, green fluorescent protein; MOI, multiplicity of infection.