FIG. 5.

Stable engraftment and repopulation of CD105-LV^GFP transduced human HSCs in vivo. Six-weeks-old NSG mice (n=9) were irradiated at 1.8 or 2 Gy. Four hours after conditioning 1.2–1.7×10⁶ CD105-LV^GFP transduced human CD34⁺ cells that were isolated from G-CSF mobilized peripheral blood were transplanted intravenously. Seven to 18 weeks post-transplantation cells were isolated from BM, spleen, and blood and analyzed by flow cytometry. (A) Lineage distribution of human CD45⁺ cells in the BM and (B) spleen. (C) Percentages of GFP⁺ cells within the engrafted human CD45⁺ cell population in BM, spleen, and blood. (D) GFP expression in human CD34-purified cells 72 h post-transduction (pretransplantation) and in engrafted human CD45⁺ cells (7–18 weeks post-transplantation) in BM and spleen; ns, not significant. Comparable percentages of GFP⁺ cells in each lineage in human CD45⁺ engrafted (E) BM and (F) spleen cells; differences not significant according to one-way ANOVA analysis: P=0.2752 (BM), P=0.1254 (spleen). BM, bone marrow; h, human; NSG, NOD-scid IL2Rγ^−/−.