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. 2015 Mar 11;56(3):1719–1732. doi: 10.1167/iovs.14-16087

Figure 5.

Figure 5

TLR2- and MyD88-deficient eyes exhibit reduced CRAMP levels. Eyes (n = 4) of WT (C57BL/6), TLR2−/−, and MyD88−/− mice (both on B6 background) were infected with S. aureus (5000 CFU/eye of strain RN6390). CRAMP expression in the retina (24 hours post infection) was detected by immunostaining (blue, DAPI nuclear stain; green, CRAMP+ve cells) (A) and dot-blot (C). For quantitative analysis, qRT-PCR was performed at the indicated time points to assess mRNA levels of CRAMP (B). Dots were quantitated by densitometric analysis and are presented as fold increases in comparison to uninfected controls. Student's t-test was used for statistical analysis, and comparisons were made between WT versus TLR2−/− (*) and WT versus MyD88−/− (#); (*#P < 0.05; **##P < 0.005). Magnification: ×20.